Publications

Yoshiaki Yasutake website

Publications

  • Yasutake Y, Hattori SI, Kumamoto H, Tamura N, Maeda K & Mitsuya H. (2024)  Deviated binding of anti-HBV nucleoside analog E-CFCP-TP to the reverse transcriptase active site attenuates the effect of drug-resistant mutations. Sci. Rep., 14, 15742. [PubMed] [Scientific Reports] [PDB codes: 8x1z, 8×20, 8×21 & 8×22]
  • Hamana H, Yasutake Y, Kato-Murayama M, Hosaka T, Shirouzu M, Sakasegawa S, Sugimori D & Murayama K. (2023)  Structural basis for the substrate specificity switching of lysoplasmalogen-specific phospholipase D from Thermocrispum sp. RD004668. Biosci. Biotechnol. Biochem., 87(1), 74-81. [PubMed] [PDB codes: 7ymp, 7ymq, 7ymr & 7ym0]
  • Konishi K, Yasutake Y, Muramatsu S, Murata S, Yoshida K, Ishiya K, Aburatani S, Sakasegawa S & Tamura T. (2022)  Disruption of SMC-related genes promotes recombinant cholesterol esterase production in Burkholderia stabilis. Appl. Microbiol. Biotechnol., 106, 8093-8110. [PubMed] [AMB]
  • Yamamoto K, Yamamoto N, Ayukawa S, Yasutake Y, Ishiya K & Nakashima N. (2022)  Scaffold size-dependent effect on the enhanced uptake of antibiotics and other compounds by Escherichia coli and Pseudomonas aeruginosa. Sci. Rep., 12, 5609. [PubMed] [Scientific Reports]
  • Nakajima S, Watashi K, Kato T, Muramatsu M, Wakita T, Tamura N, Hattori SI, Maeda K, Mitsuya H, Yasutake Y & Toyoda T. (2021)  Biochemical and structural properties of entecavir-resistant hepatitis B virus polymerase with L180M/M204V mutations. J. Virol., 95(16), e02401-20. [PubMed] [ASM] [PDB codes: 7dbm & 7dbn]
  • Yasutake Y, Konishi K, Muramatsu S, Yoshida K, Aburatani S, Sakasegawa S & Tamura T. (2021)  Bacterial triacylglycerol lipase is a potential cholesterol esterase: identification of a key determinant for sterol-binding specificity. Int. J. Biol. Macromol., 167, 578-586. [PubMed] [ScienceDirect] [PDB codes: 7cof & 7cog]
  • Mie Y, Yasutake Y, Takayama H & Tamura T. (2020)  Electrochemically boosted cytochrome P450 reaction that efficiently produces 25-hydroxyvitamin D3. J. Catal., 384, 30-36. [ScienceDirect}
  • Yasutake Y, Hattori SI, Tamura N, Matsuda K, Kohgo S, Maeda K & Mitsuya H. (2020)  Structural features in common of HBV and HIV-1 resistance against chirally-distinct nucleoside analogues entecavir and lamivudine. Sci. Rep., 10, 3021. [PubMed] [Scientific Reports] [PDB codes: 6kdj, 6kdk, 6kdm, 6kdn & 6kdo]
  • Usuki S, Yasutake Y, Tamura N, Tamura T, Tanji K, Saitoh T, Murai Y, Mikami D, Yuyama K, Monde K, Mukai K & Igarashi Y. (2020)  Nrp1 is activated by konjac ceramide binding-induced structural rigidification of the a1a2 domain. Cells, 9(2), 517. [PubMed]
  • Yoshida K, Konishi K, Magana-Mora A, Rougny A, Yasutake Y, Muramatsu S, Murata S, Kumagai T, Aburatani S, Sakasegawa S & Tamura T. (2019)  Production of recombinant extracellular cholesterol esterase using consistently active promoters in Burkholderia stabilis. Biosci. Biotechnol. Biochem., 83(10), 1974-1984. [PubMed]
  • Saito Y, Kitagawa W, Kumagai T, Tajima N, Nishimiya Y, Tamano K, Yasutake Y, Tamura T & Kameda T. (2019) Developing a codon optimization method for improved expression of recombinant proteins in actinobacteria. Sci. Rep., 9, 8338. [PubMed] [Press release]
  • Mie Y, Yasutake Y, Ikegami M & Tamura T. (2019) Anodized gold surface enables mediator-free and low-overpotential electrochemical oxidation of NADH: A facile method for the development of an NAD+-dependent enzyme biosensor. Sensor. Actuat. B-Chem., 288, 512-518. [ScienceDirect]
  • Yasutake Y, Hattori SI, Tamura N, Matsuda K, Kohgo S, Maeda K & Mitsuya H. (2019) Active-site deformation in the structure of HIV-1 RT with HBV-associated septuple amino acid substitutions rationalizes the differential susceptibility of HIV-1 and HBV against 4ʹ-modified nucleoside RT inhibitors. Biochem. Biophys. Res. Commun., 509(4), 943-948. [PubMed] [PDB codes: 6ik9 & 6ika]
  • Yoshida K, Yasutake Y & Tamura T. (2018) Complete genome sequence of an efficient vitamin D3-hydroxylating bacterium, Pseudonocardia autotrophica NBRC 12743. Microbiol. Resour. Announc., 7(12), e01105-18. [PubMed] [GenBank codes: AP018920 & AP018921; SRA codes: DRX139961 & DRX139962]
  • Yasutake Y, Hattori SI, Hayashi H, Matsuda K, Tamura N, Kohgo S, Maeda K & Mitsuya H. (2018) HIV-1 with HBV-associated Q151M substitution in RT becomes highly susceptible to entecavir: structural insights into HBV-RT inhibition by entecavir. Sci. Rep., 8, 1624. [PubMed] [PDB codes: 5xn0, 5xn1 & 5xn2][Press release]
  • Yasutake Y, Kusada H, Ebuchi T, Hanada S, Kamagata Y, Tamura T & Kimura N. (2017) Bifunctional quorum-quenching and antibiotic-acylase MacQ forms a 170-kDa capsule-shaped molecule containing spacer polypeptides. Sci. Rep., 7, 8946. [PubMed] [PDB codes: 4yf9, 4yfa, 4yfb & 5c9i]
  • Yasutake Y, Kameda T & Tamura T. (2017) Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D3 hydroxylase (CYP107BR1) caused by a mutation distant from the active site. Acta Crystallogr. Sect. F, 73(5), 266-275. [PubMed] [PDB codes: 5gnl & 5gnm]
  • Nakamura A, Tamura N & Yasutake Y. (2015) Structure of the HIV-1 reverse transcriptase Q151M mutant: insights into the inhibitor resistance of HIV-1 reverse transcriptase and the structure of the nucleotide-binding pocket of Hepatitis B virus polymerase. Acta Crystallogr. Sect. F, 71(11), 1384-1390. [PubMed] [PDB code: 4zhr]
  • Matsumoto Y, Yasutake Y, Takeda Y, Tamura T, Yokota A & Wada M. (2015) Structural insights into the substrate stereospecificity of D–threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23: a useful enzyme for the synthesis of optically pure L–threo– and of D–erythro-3-hydroxyaspartate. Appl. Microbiol. Biotechnol., 99(17), 7137-7150. [PubMed] [PDB codes: 3wqc, 3wqd, 3wqe, 3wqf, 3wqg, 4pb3, 4pb4 & 4pb5] 
  • Enomoto M, Kitagawa W, Yasutake Y & Shimizu H. (2014) Total synthesis of aurachins C, D and L, and a structurally simplified analog of aurachin C. Biosci. Biotechnol. Biochem., 78(8), 1324-1327. [PubMed]
  • Yasutake Y,  Kitagawa W, Hata M, Nishioka T, Ozaki T, Nishiyama M, Kuzuyama T & Tamura T. (2014) Structure of the quinoline N-hydroxylating cytochrome P450 RauA, an essential enzyme that confers antibiotic activity on aurachin alkaloids. FEBS Lett., 588(1), 105-110.  [PubMed] [PDB code: 3wec]
  • Yasutake Y,  Nishioka T, Imoto N & Tamura T. (2013) A single mutation at the ferredoxin binding site of P450 Vdh enables efficient biocatalytic production of 25-hydroxyvitamin D3. ChemBioChem, 14(17), 2284-2291. [PubMed] [PDB code: 3vrm] [Recommended in F1000Prime]
  • Matsumoto Y, Yasutake Y, Takeda Y, Tamura T, Yokota A & Wada M. (2013) Crystallization and preliminary X-ray diffraction studies of D–threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23. Acta Crystallogr. Sect. F, 69(10), 1131-1134.  [PubMed]
  • Kitagawa W, Ozaki T, Nishioka T, Yasutake Y, Hata M, Nishiyama M, Kuzuyama T & Tamura T. (2013) Cloning and heterologous expression of the aurachin RE biosynthesis gene cluster afford a novel cytochrome P450 for quinoline N-hydroxylation. ChemBioChem, 14(9), 1085-1093. [PubMed]
  • Kitahara K, Yasutake Y & Miyazaki K. (2012) Mutational robustness of 16S ribosomal RNA, shown by experimental horizontal gene transfer in Escherichiacoli. Proc. Natl. Acad. Sci. USA, 109(47), 19220-19225. [PubMed] [Press release]
  • Takeuchi J, Maehashi K, Yasutake Y, Muramatsu Y, Miyata K, Watanabe T & Nagashima T. (2012) Properties of emu (Dromaius novaehollandiae) albumen proteins. Food Res. Int., 49(1), 567-571. [ScienceDirect]
  • Nishioka T, Yasutake Y, Nishiya Y & Tamura T. (2012) Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV. FEBS J., 279(17), 3264-3275. [PubMed] [PDB codes: 3aus, 3aut, 3auu, 3ay6 & 3ay7]
  • Yasutake Y, Ota H, Hino E, Sakasegawa S & Tamura T. (2011) Structures of Burkholderia thailandensis nucleoside kinase: implications for the catalytic mechanism and nucleoside selectivity. Acta Crystallogr. Sect. D, 67(11), 945-956. [PubMed] [PDB codes: 3b1o, 3b1n, 3b1p, 3b1q & 3b1r]
  • Yasutake Y,  Fujii Y, Nishioka T, Cheon W-K, Arisawa A & Tamura T. (2010) Structural evidence for the enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase. J. Biol. Chem., 285(41), 31193-31201. [PubMed] [PDB codes: 3a4g, 3a4h, 3a4z, 3a50 & 3a51]
  • Yasutake Y,  Fujii Y, Nishioka T, Cheon W-K, Arisawa A & Tamura T. (2009) Crystallization and preliminary X-ray diffraction studies of vitamin D3 hydroxylase, a novel cytochrome P450 isolated from Pseudonocardia autotrophica. Acta Crystallogr. Sect. F, 65(4), 372-375. [PubMed]
  • Nishioka T, Yasutake Y, Nishiya Y, Tamura N & Tamura T. (2009) C-terminal tail derived from the neighboring subunit is critical for the activity of ThermoplasmaacidophilumD-aldohexose dehydrogenase. Proteins, 74(4), 801-807. [PubMed] [PDB code: 2zk7]
  • Yasutake Y, Imoto N, Fujii Y, Fujii T, Arisawa A & Tamura T. (2007) Crystal structure of cytochrome P450 MoxA from Nonomuraea recticatena (CYP105). Biochem. Biophys. Res. Commun., 361(4), 876-882. [PubMed] [PDB code: 2z36]
  • Tanaka Y, Kuroda M, Yasutake Y, Yao M, Tsumoto K, Watanabe N, Ohta T & Tanaka I. (2007) Crystal structure analysis reveals a novel forkhead-associated domain of ESAT-6 secretion system C protein in Staphylococcus aureus. Proteins, 69(3), 659-664. [PubMed] [PDB code: 1wv3]
  • Tanaka Y, Sasaki T, Kumagai I, Yasutake Y, Yao M, Tanaka I & Tsumoto K. (2007) Molecular properties of two proteins homologous to PduO-type ATP:cob(I)alamin adenosyltransferase from Sulfolobustokodaii. Proteins, 68(2), 446-457. [PubMed] [PDB codes: 1woz & 1wvt]
  • Yasutake Y, Nishiya Y, Tamura N & Tamura T. (2007) Structural insights into unique substrate selectivity of Thermoplasma acidophilumD-aldohexose dehydrogenase. J. Mol. Biol., 367(4), 1034-1046. [PubMed] [PDB codes: 2dtd, 2dte & 2dtx]
  • Yasutake Y, Nishiya Y, Tamura N & Tamura T. (2006) Crystallization and preliminary crystallographic analyses of NAD-preferring aldohexose dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum. Acta Crystallogr. Sect. F, 62(6), 586-589. [PubMed]
  • Yasutake Y, Kawano S, Tajima K, Satoh Y, Yao M, Munekata M & Tanaka I. (2006) Structural characterization of the Acetobacter xylinum endo-β-1,4-glucanse CMCax required for cellulose biosynthesis. Proteins, 64(4), 1069-1077. [PubMed] [PDB code: 1wzz]
  • Tanaka Y, Tsumoto K, Tanabe E, Yasutake Y, Sakai N, Yao M, Tanaka I & Kumagai I. (2005) Crystal structure of hypothetical protein ST2072 from Sulfolobus tokodaii. Proteins, 61, 1127-1131. [PubMed] [PDB code: 1ve0]
  • Watanabe S, Yasutake Y, Tanaka I & Takada Y. (2005) Elucidation of stability determinants of cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Colwellia maris, by construction of chimeric enzymes. Microbiology, 151(4), 1083-1094. [PubMed]
  • Yao M, Yasutake Y, Morita H & Tanaka I. (2005) Structure of the type I L-asparaginase from the hyperthermophilic archaea Pyrococcus horikoshii at 2.16 Å resolution. Acta Crystallogr. Sect. D, 61(3), 294-301. [PubMed] [PDB code: 1wls]
  • Kawano S, Yasutake Y, Tajima K, Satoh Y, Yao M, Tanaka I & Munekata M. (2005) Crystallization and preliminary crystallographic analysis of the cellulose biosynthesis-related protein CMCax from Acetobacter xylinum. Acta Crystallogr. Sect. F, 61(2), 252-254. [PubMed]
  • Yasutake Y, Yao M, Sakai N, Kirita T & Tanaka I. (2004) Crystal structure of the Pyrococcus horikoshii isopropylmalate isomerase small subunit provides insight into the dual substrate specificity of the enzyme. J. Mol. Biol., 344(2), 325-333. [PubMed] [PDB code: 1v7l]
  • Tanaka Y, Tsumoto K, Umetsu M, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Arakawa T & Kumagai I.  (2004) Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride. Biochem. Biophys. Res. Commun., 323(1), 185-191. [PubMed] [PDB code: 1umj]
  • Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao M, Tanaka I, Fukada H & Kumagai I. (2004) How oligomerization contributes to the thermostability of an archaeon protein: protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii. J. Biol. Chem., 279(31), 32957-32967. [PubMed] [PDB code: 1vbf]
  • Tanaka Y, Tsumoto K, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I & Kumagai I. (2004) Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA. FEBS Lett., 556(1-3), 167-174. [PubMed] [PDB codes: 1j2v & 1uku]
  • Yao M, Yasutake Y & Tanaka I. (2004) Flash-cooling of macromolecular crystals in a capillary to overcome increased mosaicity. Acta Crystallogr. Sect.  D, 60(1), 39-45. [PubMed]
  • Yasutake Y, Watanabe S, Yao M, Takada Y, Fukunaga N & Tanaka I. (2003) Crystal structure of the monomeric isocitrate dehydrogenase in the presence of NADP: Insight into the cofactor recognition, catalysis, and evolution. J. Biol. Chem., 278(38), 36897-36904. [PubMed] [PDB code: 1j1w]
  • Yasutake Y, Watanabe S, Yao M, Takada Y, Fukunaga N & Tanaka I. (2002) Structure of the monomeric isocitrate dehydrogenase: evidence of a protein monomerization by a domain duplication. Structure, 10(12), 1637-1648. [PubMed] [PDB code: 1itw]
  • Yasutake Y, Watanabe S, Yao M, Takada Y, Fukunaga N & Tanaka I. (2001) Crystallization and preliminary X-ray diffraction studies of monomeric isocitrate dehydrogenase by the MAD method using Mn atoms. Acta Crystallogr. Sect. D, 57(11), 1682-1685. [PubMed]

和文総説等

  • 小西健司、酒瀬川信一、村松周治、安武義晃、田村具博 (2024) 「コレステロールエステラーゼ大量生産スマートセルの開発」バイオサイエンスとインダストリー 82(2), 238-241
  • 三重安弘、安武義晃 (2020) 「ナノ構造電極による酸化還元酵素の活性制御」バイオサイエンスとインダストリー 78(6), 496-497
  • 酒瀬川信一、村田里美、小西健司、吉田圭太朗、安武義晃、油谷幸代、田村具博 (2018) 「診断薬用酵素コレステロールエステラーゼ （CEN）生産への応用」スマートセルインダストリー〜微生物細胞を用いた物質生産の展望〜（シー・エム・シー出版）
  • 亀田倫史、齋藤裕、田島直幸、西宮佳志、玉野孝一、北川航、安武義晃、田村具博 (2018) 「情報解析に基づく遺伝子配列改変による発現量調節」スマートセルインダストリー〜微生物細胞を用いた物質生産の展望〜（シー・エム・シー出版）
  • 和田大、安武義晃 (2014)「反応に金属を要求するPLP酵素」化学と生物 52(10), 634-636
  • 安武義晃 (2014)「多彩にして多才：シトクロムP450」日本の結晶学 (II) -その輝かしい発展- 350（日本結晶学会）
  • 安武義晃、田村具博 (2011)「微生物変換によるビタミンD3の効率的生産〜分子の改良から細胞膜改変までの包括的アプローチ〜」シンセシオロジー 4(4), 222-229
  • 安武義晃、姚閔、渡邉信久、田中勲 (2002)「タンパク質結晶構造解析の現状」生化学 74 (10), 1272-1278
  • 安武義晃、姚閔、田中勲 (2002)「ハイスループットX線結晶構造解析」蛋白質 核酸 酵素47 (8), 1033-1037