近年、 がん等の疾病と金属蛋白質との関連が明らかになってきており、その蛋白質機能・特性を分子レベルで理解することは、該蛋白質をターゲットとする創薬において重要となっています。しかしながら、機能発現に重要な金属の酸化還元（レドックス）状態を制御する良好な手法が確立されておらず、in vivoでの疾病との相関研究に比べその理解は遅れています。本研究では、前述のナノ構造電極を活用する電気化学法を用いて、創薬ターゲットとなる金属蛋白質のレドックス制御を行いながら、基質や阻害剤などとの反応を調べ該蛋白質機能の分子レベルでの理解を進展させることを目指しています。

1. Mie, Y., Takayama, H., Hirano, Y., Facile Control of Surface Crystallographic Orientation of Anodized Nanoporous Gold Catalyst and its Application for Highly Efficient Hydrogen Evolution Reaction, J. Catal., in press.
2. Mie, Y., Takahashi K., Torii R., Jingkai S, Tanaka T, Sueyoshi K, Tsujino H, Yamashita T. Redox State Control of Human Cytoglobin by Direct Electrochemical Method to Investigate its Function in Molecular Basis.Chem Pharm Bull (Tokyo), doi:10.1248/cpb.c20-00175 (2020).
3. Mie, Y., Yasutake, Y., Takayama, H., Tamura, T. Electrochemically boosted cytochrome P450 reaction that efficiently produces 25-hydroxyvitamin D-3. Journal of Catalysis 384, 30-36, doi:10.1016/j.jcat.2020.02.012 (2020).
4. Mie, Y., Takahashi, K., Itoga, Y., Sueyoshi, K., Tsujino, H., Yamashita, T. Nanoporous gold based electrodes for electrochemical studies of human neuroglobin. Electrochemistry Communications 110, doi:10.1016/j.elecom.2019.106621 (2020).
5. Mie, Y., Yasutake, Y., Ikegami, M. and Tamura, T. Anodized gold surface enables mediator-free and low-overpotential electrochemical oxidation of NADH: A facile method for the development of an NAD(+)-dependent enzyme biosensor. SENSORS AND ACTUATORS B-CHEMICAL, 288, 512-518 doi:10.1016/j.snb.2019.03.039 (2019).
6. Mie, Y., Suzuki, M. and Komatsu, Y. Electrochemically-driven drug metabolism reaction by membrane containing human cytochrome P450. J. Am. Chem. Soc., 131,6646-6647(2009).
7. Mie, Y., Ikegami, M. and Komatsu, Y. Gold sputtered electrode surfaces enhance direct electron transfer reactions of human cytochrome P450s. Electrochm. Commun., 12, 680-683 (2010).
8. Ikegami, M., Mie, Y. and Komatsu, Y. Size-controlled fabrication of gold nanodome arrays and its application to enzyme electrodes.Colloids and Surfaces A: Physicochemical and Engineering Aspects, 384, 388-392 (2011).
9. Ikegami, M., Mie, Y., Hirano, Y. and Komatsu, Y. Direct Electrochemistry of Microsomal Human Flavin-containing Monooxygenases 1 and 3 on Naphthalenethiol Thin Films. Ecs Electrochemistry Letters, 2, G5-G7 (2013).
10. Mie, Y., Tateyama, E. and Komatsu, Y. p-Aminothiophenol modification on gold surface improves stability for electrochemically driven cytochrome P450 microsome activity. Electrochim. Acta, 115, 364-369 (2014).
11. Mie, Y., Ikegami, M. and Komatsu, Y. Nanoporous structure of gold electrode fabricated by anodization and its efficacy for direct electrochemistry of human cytochrome P450. Chem. Lett., 45,640-642 (2016).