Electrochemical control of enzyme catalysis and evaluation of cellular reactios


Cytochrome P450 (CYP) receives electrons to catalyze monooxygenation for a variety of substrates. We are developing the electron supplying method from an electrode to CYP immobilized on it, in order to monitor or utilize the CYP catalysis. We have proven that the nanostructured electrode surface (e.g. nanoporous gold) promotes the efficient electron transfer of the enzyme. We are seeking more effective electrodes and CYP-immobilization techniques.

We also focus on single-cell analyses, and have developed scanning electrochemical microscopy (SECM) enabling long-term monitoring of cellular topography or activities. We are further developing a novel SECM system to carry out single-cell analysis more conveniently.

Electrochemically-driven CYP reaction and cell kinetics observation by SECM.