Chemical linkers for post-synthetic modifications of oligonucleotides


Chemical modifications of oligonucleotides are frequently for genetic analyses or oligonucleotide therapeutics. Chemical linkers are essential for covalent bonding between oligonucleotides and exogenous molecules. We have developed highly reactive amino-linkers for terminal modifications of oligonucleotides. These linkers enable high-throughput purification and high-yield modifications of functional oligonucleotides. We have also developed interstrand cross-linkers that can site-specifically conjugate a complementary duplex. Cross-linked duplexes form extraordinarily stable helices, enabling enzyme immobilization on electrode and construction of therapeutic agents.

Terminal modification and interstrand cross-link of oligonucleotids.